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@@ -402,7 +402,7 @@ impl Sam {
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if is_reverse(flag) {
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let len = ref_range.len();
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ref_range.end = (ref_pos as usize) + 1;
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- ref_range.start = ref_pos as usize + len - 1; // wo -1 panic at 501 make_aln
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+ ref_range.start = ref_pos as usize + len - 2; // wo -1 panic at 501 make_aln
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query_range.end = query_range.end - 1;
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} else {
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let len = ref_range.len();
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