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@@ -391,7 +391,7 @@ impl Sam {
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if is_reverse(flag) {
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let len = ref_range.len();
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ref_range.end = (ref_pos as usize) - 1;
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- ref_range.start = ref_pos as usize + len - 1;
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+ ref_range.start = ref_pos as usize + len - 2;
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query_range.end = query_range.end + 1;
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} else {
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let len = ref_range.len();
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