/* * Require in path : zgrep, grep */ import { spawn } from 'child_process'; import os from 'os'; import path from 'path' import fs from 'fs' const async_exec = (prog: string, args: string[], onData: Function, onErr: Function) => { return new Promise((resolve, reject) => { const child = spawn(prog, args, {shell: true}) child.stdout.on('data', data => onData(data.toString().trim())) child.stderr.on('data', data => onErr(data.toString().trim())) child.on('error', err => reject(err)) child.on('exit', code => resolve(code)) }) } const rmList = (list: string[]) => { return Promise.all(list.map(e => fs.promises.rm(e, {recursive: true}))) } const denovoAssemblage = ( reads : string | string[], rnames: string[], spadesPath: string ) => { return new Promise(async (resolve, reject) => { let readsIn: string[] = [] let isPairedEnd = false const threads = String(os.cpus().length) if (Array.isArray(reads)) { if (reads.length > 2) { reject('Only R1 and R2 path are required') } else { console.log('Assuming paired end reads'); isPairedEnd = true readsIn = reads } } else { readsIn = [reads] } // Prepare the fastq files for spades inputs const rnamesSel = '\'' + rnames.join('\\|') + '\'' const tmpSubReads: string[] = [] for (const R of readsIn) { const tmp = path.join(os.tmpdir(), (+new Date) + '_' + path.parse(R).base) tmpSubReads.push(tmp) const greper = R.match(/gz$/) ? 'zgrep' : 'grep' await async_exec(greper, [rnamesSel, R, '-A3', '--no-group-separator', '>', tmp], console.log, console.log) } const args = isPairedEnd ? ['-1', tmpSubReads[0], '-2', tmpSubReads[1]] : ['-s', tmpSubReads[0]] const rsltDir = path.join(os.tmpdir(), (+new Date) + '_spades') await async_exec(spadesPath, ['-t', threads, '--isolate', ...args, '-o', rsltDir], console.log, console.log) if(!fs.existsSync(path.join(rsltDir, 'contigs.fasta'))) { await rmList([rsltDir]) console.log('Trying meta') await async_exec(spadesPath, ['-t', threads, '--meta', ...args, '-o', rsltDir], console.log, console.log) if(!fs.existsSync(path.join(rsltDir, 'contigs.fasta'))) { await rmList([rsltDir, ...tmpSubReads]) reject('No convergence') } } else { await rmList([rsltDir, ...tmpSubReads]) reject('No convergence') } await rmList(tmpSubReads) resolve(rsltDir) }) } export { denovoAssemblage } /* (async()=>{ const spadesPath = '/home/thomas/NGS/tools/SPAdes-3.15.0-Linux/bin/spades.py' const reads2 = '/home/thomas/Documents/Programmes/ttest/71-mer.fa' const rnames = ['33530080_3', '30971394_3', '77190111_3', '89825138_3', '22481866_3', '111937620_4', '24308941_6', '27758147_3', '87242990_14', '41688638_4', '114699822_3', '48573844_4', '91080996_3', '99644261_3', '77207124_3', '28986564_10', '84400117_4', '10884880_6', '116082011_12', '1739367_3', '13550404_3', '68446023_10', '50560660_3', '9046992_3'] console.log(await denovoAssemblage(reads2, rnames, spadesPath)); })() */