Thomas 1 рік тому
батько
коміт
e2cff587e4
13 змінених файлів з 25812 додано та 291 видалено
  1. 788 46
      Cargo.lock
  2. 8 0
      Cargo.toml
  3. 66 3
      src/genomic_graph.rs
  4. 291 242
      src/lib.rs
  5. 209 0
      src/phase.rs
  6. 3847 0
      test.bed
  7. 1948 0
      test_1.bed
  8. 8975 0
      test_2.bed
  9. 4208 0
      test_3.bed
  10. 3617 0
      test_4.bed
  11. 1852 0
      test_5.bed
  12. 3 0
      test_6.bed
  13. BIN
      test_chr1.bytes.gz

Різницю між файлами не показано, бо вона завелика
+ 788 - 46
Cargo.lock


+ 8 - 0
Cargo.toml

@@ -12,11 +12,19 @@ aligner_client = { git = "https://git.t0m4.fr/Thomas/aligner_client.git"}
 rust-htslib = "0.46.0"
 anyhow = "1.0.75"
 log = "0.4.19"
+rand = "0.8.4"
 uuid = { version = "1.6.1", features = ["serde", "v4"] }
 seq_io = "0.3.2"
 noodles-fasta = "0.34.0"
+noodles-vcf = "0.51.0"
 crossbeam = "0.8.4"
+pileup = { git = "https://git.t0m4.fr/Thomas/pileup.git" }
+libVariant = { git = "https://git.t0m4.fr/Thomas/libVariant.git" }
 crossbeam-channel = "0.5.12"
 test-log = "0.2.15"
 num-format = "0.4.4"
 petgraph = "0.6.4"
+indicatif = {version = "0.17.8", features = ["rayon"]}
+indicatif-log-bridge = "0.2.2"
+rayon = "1.8.0"
+dashmap = "5.5.3"

+ 66 - 3
src/genomic_graph.rs

@@ -2,7 +2,7 @@ use std::collections::{HashMap, HashSet};
 
 use petgraph::{
     dot::Dot,
-    stable_graph::{NodeIndex, StableGraph},
+    stable_graph::{NodeIndex, StableGraph, EdgeIndex},
 };
 
 use crate::{breakpoint::BreakPoint, Genome};
@@ -112,7 +112,11 @@ impl GenomicGraph {
         &mut self,
         start: (bool, &str, i32),
         end: (bool, &str, i32),
-    ) -> Vec<Vec<(NodeIndex, bool, BreakPoint, String)>> {
+    ) -> (
+        GenomicGraph,
+        StableGraph<String, String>,
+        Vec<Vec<(NodeIndex, bool, BreakPoint, String)>>,
+    ) {
         // Create a new working graph.
         let mut gg = self.clone();
         // Add start and end nodes.
@@ -169,7 +173,66 @@ impl GenomicGraph {
             s.join("")
         });
 
-        ways
+        // Draw a presentable graph that integrate ways.
+        let mut integrated_graph = gg.g.map(
+            |node_id, node_val| Vec::new(),
+            |edge_id, edge_val| Vec::new(),
+        );
+        ways.iter().enumerate().for_each(|(i, way)| {
+            way.iter().for_each(|(node_id, sens, bp, s)| {
+                let v = integrated_graph.node_weight_mut(*node_id).unwrap();
+                v.push((s.to_string(), i));
+            });
+            way.windows(2).for_each(|w| {
+                let (left_node_id, left_sens, left_bp, left_s) = &w[0];
+                let (right_node_id, right_sens, right_bp, right_s) = &w[1];
+                let edge_id = integrated_graph.find_edge(*left_node_id, *right_node_id).unwrap();
+                let v = integrated_graph.edge_weight_mut(edge_id).unwrap();
+                v.push(i);
+            });
+        });
+
+        let mut change_edges: Vec<(EdgeIndex, Vec<usize>)> = Vec::new();
+        let mut integrated_graph = integrated_graph.map(
+            |node_id, node_val| {
+                if node_val.len() == 0 {
+                    return gg.g.node_weight(node_id).unwrap().str_sens(&gg.params.sens_symb, &gg.params.rc_symb);
+                }
+                let mut h: HashMap<String, Vec<usize>> = HashMap::new();
+                node_val.iter().for_each(|(k, v)| {
+                    if let Some(val) = h.get_mut(k) {
+                        val.push(*v)
+                    } else {
+                        h.insert(k.to_string(), vec![*v]);
+                    }
+                });
+                let s: Vec<String> = h.iter()
+                    .map(|(k, v)| {
+                        let mut v = v.clone();
+                        v.sort();
+                        let v = v.iter().map(|u| (u + 1).to_string()).collect::<Vec<String>>().join(", ");
+                        format!("{k} ({v})")
+                }).collect();
+                s.join("\n")
+            },
+            |edge_id, edge_val| {
+                change_edges.push((edge_id, edge_val.to_vec()));
+                "".to_string()
+            },
+        );
+
+        for (edge_id, new_edges_val) in change_edges {
+            let (left_id, right_id) = integrated_graph.edge_endpoints(edge_id).unwrap();
+            integrated_graph.remove_edge(edge_id);
+            new_edges_val.iter().for_each(|v| {
+                integrated_graph.add_edge(left_id,right_id, (v + 1).to_string());
+            })
+        }
+
+        // let dot = integrated_graph.dot_graph();
+        // println!("{dot}");
+
+        (gg, integrated_graph, ways)
     }
 }
 

+ 291 - 242
src/lib.rs

@@ -1,14 +1,15 @@
 pub mod breakpoint;
 pub mod genomic_graph;
+mod phase;
 
 use anyhow::{anyhow, Ok, Result};
-use breakpoint::BreakPoint;
 use fasta::record::Sequence;
 use log::info;
 use minimap2::{Aligner, Mapping};
 use noodles_fasta as fasta;
 use num_format::{CustomFormat, Grouping, ToFormattedString, WriteFormatted};
 use petgraph::{dot::Dot, prelude::*};
+use rayon::prelude::*;
 use rust_htslib::bam::{self, Record};
 use std::{
     collections::HashMap,
@@ -987,87 +988,28 @@ pub fn dot_graph_biall(
     dot
 }
 
-fn get_next(
-    graph: &StableGraph<BreakPoint, String>,
-    ways: Vec<Vec<(NodeIndex, bool)>>,
-) -> Vec<Vec<(NodeIndex, bool)>> {
-    let mut visited = Vec::new();
-    for way in ways.into_iter() {
-        let mut possible_next: Vec<(NodeIndex, bool)> = Vec::new();
-        let (last_id, last_sens) = way.get(way.len() - 1).unwrap().to_owned();
-        let a = graph.node_weight(last_id).unwrap().to_owned();
-        let last = if last_sens { a.clone() } else { a.rc() };
-
-        // Check for every neighbors if they can be visited
-        for neighbor_id in graph.neighbors(last_id) {
-            let b = graph.node_weight(neighbor_id).unwrap().to_owned();
-            if last.is_next(&b) {
-                possible_next.push((neighbor_id, true));
-            }
-            if last.is_next(&b.rc()) {
-                possible_next.push((neighbor_id, false));
-            }
-        }
-
-        // If no visitable neighbors continue to the next way
-        if possible_next.len() == 0 {
-            continue;
-        }
-        for pn in possible_next {
-            // If the possible next node in the way isn't already visited add it as visited
-            let mut new_way = way.clone();
-            if !new_way
-                .iter()
-                .map(|(id, _)| id.clone())
-                .collect::<Vec<NodeIndex>>()
-                .contains(&pn.0)
-            {
-                new_way.push(pn);
-                visited.push(new_way);
-            } else {
-                // println!("dropping way: {way:?} next : {:?}", pn);
-            }
-        }
-    }
-    visited
-}
-
-pub fn find_ways(
-    graph: &StableGraph<BreakPoint, String>,
-    start_id: NodeIndex,
-    start_sens: bool,
-    end_id: NodeIndex,
-) -> Vec<Vec<(NodeIndex, bool)>> {
-    let mut reached_end = Vec::new();
-    let mut ways = vec![vec![(start_id, start_sens)]];
-    loop {
-        let new_ways = get_next(graph, ways.clone());
-        if new_ways.len() == 0 {
-            break;
-        }
-
-        ways.clear();
-        for new_way in new_ways.iter() {
-            let last_id = new_way.last().unwrap().to_owned().0;
-            if last_id == end_id {
-                reached_end.push(new_way.clone());
-            } else {
-                ways.push(new_way.clone());
-            }
-        }
-    }
-    reached_end
-}
-
 #[cfg(test)]
 mod tests {
-    use std::collections::HashSet;
-
-    use petgraph::{stable_graph::StableGraph, visit::EdgeRef, Directed};
-
-    use crate::genomic_graph::GenomicGraph;
+    use std::{
+        slice::Windows,
+        sync::{mpsc::channel, Arc},
+    };
+
+    use dashmap::DashSet;
+    use indicatif::MultiProgress;
+    use indicatif_log_bridge::LogWrapper;
+    use libVariant::{
+        utils::{new_pg, new_pg_speed},
+        variants::{Variant, VariantCategory},
+    };
+    use pileup::records_at_base;
+    use rand::distributions::{Distribution, Uniform};
 
     use super::*;
+    use crate::{
+        genomic_graph::GenomicGraph,
+        phase::{HeteroVar, Phase},
+    };
 
     #[test]
     fn it_works() -> Result<()> {
@@ -1096,191 +1038,298 @@ mod tests {
     }
 
     #[test]
-    fn linear() -> Result<()> {
+    fn test_graph() -> Result<()> {
         let dir = "./data_test";
-        let sens_symb = "►";
-        let rc_symb = "◄";
+        let start = (true, "chr7", 0);
+        let end = (true, "chr7", i32::MAX);
+        // let end = (false, "chr15", 0);
 
         // Load from fasta in dir.
         let genome = Genome::from_contigs_sequences(dir)?;
+        let mut genomic_graph = GenomicGraph::from_genome(&genome);
 
-        // start node
-        let start_bp = BreakPoint::from(true, "chr7", 0, "start");
-        // end node
-        let end_bp = BreakPoint::from(true, "chr7", i32::MAX, "end");
-
-        // Load breakpoints as graph nodes.
-        let mut breakpoints_id = HashMap::new();
-        let mut graph = StableGraph::<BreakPoint, String, Directed>::new();
-        let mut dedup_hash = HashSet::new();
-        for contig in genome.contigs() {
-            if let Some(mappings) = contig.contig_ref.breakpoints() {
-                let bp = BreakPoint::new(mappings);
-                if !dedup_hash.contains(&bp.str_sens(sens_symb, rc_symb)) {
-                    let id = graph.add_node(bp.clone());
-                    breakpoints_id.insert(bp.id.clone(), id);
-                    dedup_hash.insert(bp.str_sens(sens_symb, rc_symb));
-                    dedup_hash.insert(bp.str_rc(sens_symb, rc_symb));
-                }
-            }
-        }
-
-        // Add start and end nodes.
-        let start_id = graph.add_node(start_bp.clone());
-        breakpoints_id.insert(start_bp.id, start_id);
+        let (oriented_graph, integrated_graph, ways) = genomic_graph.ways(start, end);
+        let dot = oriented_graph.dot_graph();
+        println!("{dot}");
 
-        let end_id = graph.add_node(end_bp.clone());
-        breakpoints_id.insert(end_bp.id, end_id);
+        let s = Dot::new(&integrated_graph).to_string().replace("\\\"", "");
+        let x11_colors: Vec<String> = vec![
+            String::from("Red"),
+            String::from("Green"),
+            String::from("Blue"),
+            String::from("Cyan"),
+            String::from("Magenta"),
+            String::from("Yellow"),
+            String::from("DarkRed"),
+            String::from("DarkGreen"),
+            String::from("DarkBlue"),
+            String::from("DarkCyan"),
+            String::from("DarkMagenta"),
+            String::from("DarkYellow"),
+            String::from("LightRed"),
+            String::from("LightGreen"),
+            String::from("LightBlue"),
+            String::from("LightCyan"),
+            String::from("LightMagenta"),
+            String::from("LightYellow"),
+            String::from("Orange"),
+            String::from("Brown"),
+            String::from("Beige"),
+        ];
+        let mut s = s.clone();
+        ways.iter().enumerate().for_each(|(i, _)| {
+            s = s.replace(
+                &format!("[ label = \"{}\" ]", i + 1),
+                &format!(
+                    "[ label = \"{}\" color = \"{}\" ]",
+                    i + 1,
+                    x11_colors[i].to_string()
+                ),
+            );
+        });
+        println!("{s}");
 
-        // Draw edges between nodes.
-        let g_iter = graph.clone();
-        for last in g_iter.node_weights() {
-            let a = *breakpoints_id.get(&last.id).unwrap();
-            for next in g_iter.node_weights() {
-                if last == next {
-                    continue;
-                }
-                let b = *breakpoints_id.get(&next.id).unwrap();
+        for (i, way) in ways.iter().enumerate() {
+            let s = way
+                .iter()
+                .map(|(_, _, _, s)| s.to_string())
+                .collect::<Vec<String>>()
+                .join("");
+            println!("{}.\t{s}", i + 1);
+        }
+        Ok(())
+    }
 
-                // Don't add edge between start and end nodes.
-                if a == start_id && b == end_id || a == end_id && b == start_id {
-                    continue;
-                }
+    #[test]
+    fn phasing() -> Result<()> {
+        let name = "CAMARA";
+        let min_records = 1;
+        let format = CustomFormat::builder()
+            .grouping(Grouping::Standard)
+            .minus_sign("-")
+            .separator("_")
+            .build()
+            .unwrap();
 
-                // A->B.
-                if last.is_next(next) || last.is_next(&next.rc()) {
-                    if graph.find_edge(a, b).is_none() {
-                        graph.add_edge(a, b, "".to_string());
+        let logger =
+            env_logger::Builder::from_env(env_logger::Env::default().default_filter_or("info"))
+                .build();
+        let multi = MultiProgress::new();
+        LogWrapper::new(multi.clone(), logger).try_init().unwrap();
+        let bam_path = &format!("/data/longreads_basic_pipe/{name}/diag/{name}_diag_hs1.bam");
+
+        let mut variants = libVariant::variants::Variants::new_from_bytes(
+            name,
+            "test_chr1.bytes.gz",
+            multi.clone(),
+        )?;
+        info!(
+            "Variants loaded {}.",
+            variants.len().to_formatted_string(&format)
+        );
+        variants
+            .data
+            .par_sort_by(|a, b| a.position.cmp(&b.position));
+
+        let pg = multi.add(new_pg_speed(variants.len() as u64));
+        pg.set_message("Phasing");
+
+        let mut phases = variants
+            .data
+            .par_chunks(2000)
+            .flat_map(|chunks| {
+                let mut bam = rust_htslib::bam::IndexedReader::from_path(bam_path).unwrap();
+                let mut errors = Vec::new();
+                let mut phases: Vec<Phase> = Vec::new();
+                for v in chunks {
+                    pg.inc(1);
+                    let mut reference = v.reference.to_string().as_bytes().to_vec();
+                    let mut altenative = v.alternative.to_string().as_bytes().to_vec();
+                    if reference.len() != 1 && altenative.len() != 1 {
+                        continue;
+                    }
+                    match HeteroVar::new(
+                        &mut bam,
+                        &v.contig.to_string(),
+                        v.position as i32,
+                        reference.pop().unwrap(),
+                        altenative.pop().unwrap(),
+                    ) {
+                        std::result::Result::Ok((a, b)) => {
+                            let mut not_merged = Vec::new();
+                            //
+                            for snp in vec![a, b].iter() {
+                                let mut has_merged = false;
+                                for phase in phases.iter_mut() {
+                                    if phase.try_merge(snp, min_records) {
+                                        has_merged = true;
+                                    }
+                                }
+                                if !has_merged {
+                                    not_merged.push(snp.clone())
+                                }
+                            }
+                            not_merged.iter().for_each(|nm| phases.push(Phase::new(nm)));
+                        }
+                        Err(e) => errors.push(e),
                     }
                 }
+                phases
+            })
+            .collect::<Vec<Phase>>();
+
+        pg.finish();
+        multi.remove(&pg);
+        // info!("before dedup {}", phases.len());
+        // phases.dedup();
+        // info!("after dedup {}", phases.len());
+        let mut n_phases = phases.len();
+        info!("Merging {} phases.", n_phases.to_formatted_string(&format));
+
+        let mut should_iter = true;
+        let plus_minus = 100;
+        let mut iter = 0;
+        let merged = Arc::new(DashSet::new());
+        let mut last_phases = phases.clone();
+
+        let pool = rayon::ThreadPoolBuilder::new().build()?;
+        pool.install(|| {
+            loop {
+                let pg = multi.add(new_pg(last_phases.len() as u64));
+                pg.set_message("Merging");
+                phases = last_phases
+                    .clone()
+                    .into_par_iter()
+                    .enumerate()
+                    .flat_map(|(x, mut phase_a)| {
+                        if !merged.contains(&x) {
+                            let pm = /* iter * plus_minus + */ plus_minus;
+                            let un_merged_ids: Vec<usize> = (0..n_phases)
+                                .into_iter()
+                                .filter(|i| !merged.contains(i))
+                                .collect();
+                            let up: Vec<_> = un_merged_ids
+                                .clone()
+                                .into_iter()
+                                .filter(|i| *i > x)
+                                .take(pm)
+                                .collect();
+                            let down: Vec<_> = un_merged_ids
+                                .into_iter()
+                                .filter(|i| *i < x)
+                                .rev()
+                                .take(pm)
+                                .collect();
+                            let range: Vec<_> = up
+                                .into_iter()
+                                .zip(down.into_iter())
+                                .flat_map(|(a, b)| vec![a, b])
+                                .collect();
+
+                            let mut n_merged = 0;
+                            range.into_iter().for_each(|y| {
+                                if !merged.contains(&y) {
+                                    let phase_b = last_phases.get(y).unwrap();
+                                    if x != y {
+                                        if phase_a.try_merge_phase(phase_b, min_records) {
+                                            n_merged += 1;
+                                            merged.insert(y);
+                                        }
+                                    }
+                                }
+                            });
+
+                            pg.inc(1);
+                            if n_merged > 0 {
+                                merged.insert(x);
+                                vec![phase_a]
+                            } else {
+                                vec![]
+                            }
+                        } else {
+                            pg.inc(1);
+                            vec![]
+                        }
+                    })
+                    .collect();
+                pg.finish();
+                multi.remove(&pg);
 
-                // If the rc of A can follow B, B->A.
-                if last.rc().is_next(&next) || last.rc().is_next(&next.rc()) {
-                    if graph.find_edge(b, a).is_none() {
-                        graph.add_edge(b, a, "".to_string());
-                    }
+                let un_merged: Vec<Phase> = (0..last_phases.len())
+                    .into_iter()
+                    .filter(|i| !merged.contains(i))
+                    .map(|i| last_phases.get(i).unwrap().to_owned())
+                    .collect();
+                info!(
+                    "Merges {}, unmerged {}, phases {}",
+                    merged.len(),
+                    un_merged.len(),
+                    last_phases.len()
+                );
+                phases.par_sort();
+                info!("before {}", phases.len());
+                phases.dedup();
+                info!("after {}", phases.len());
+                phases.extend(un_merged.into_iter());
+
+                // phases.dedup();
+                iter += 1;
+                info!("after dedup {}", phases.len());
+
+                if merged.len() == 0 {
+                    // should_iter = false;
+                    break;
+                } else {
+                    n_phases = phases.len();
+                    last_phases = phases.clone();
+                    merged.clear();
                 }
             }
-        }
-
-        // Remove nodes without edge.
-        let del: Vec<_> = graph
-            .node_indices()
-            .filter(|id| graph.neighbors_undirected(*id).count() == 0)
-            .collect();
-        del.iter().for_each(|id| {
-            graph.remove_node(*id);
-        });
-
-        let mut ways = find_ways(&graph, start_id, true, end_id);
-        ways.sort_by_key(|a| {
-            let astr: Vec<String> = a
-                .iter()
-                .map(|(id, sens)| {
-                    if *sens {
-                        graph.node_weight(*id).unwrap().str_sens(sens_symb, rc_symb)
-                    } else {
-                        graph.node_weight(*id).unwrap().str_rc(sens_symb, rc_symb)
-                    }
-                })
-                .collect();
-            astr.join(";")
-        });
-        ways.dedup_by_key(|a| {
-            let astr: Vec<String> = a
-                .iter()
-                .map(|(id, sens)| {
-                    if *sens {
-                        graph.node_weight(*id).unwrap().str_sens(sens_symb, rc_symb)
-                    } else {
-                        graph.node_weight(*id).unwrap().str_rc(sens_symb, rc_symb)
-                    }
-                })
-                .collect();
-            astr.join(";")
         });
+        info!("Final phases {}", phases.len());
+
+        let phases_len: Vec<usize> = phases.iter().map(|p| p.data.len()).collect();
+        info!(
+            "mean {}, max {}, min {}",
+            phases_len.iter().sum::<usize>() as f64 / phases_len.len() as f64,
+            phases_len.iter().max().unwrap(),
+            phases_len.iter().min().unwrap(),
+        );
 
-        // for new_way in ways.iter() {
-        //     let g = graph.map(
-        //         |ni, nv| {
-        //             let mut in_way: Vec<_> = new_way.iter().filter(|(id, _)| *id == ni).collect();
-        //             let val = if in_way.len() == 1 {
-        //                 let (_, n_sens) = in_way.pop().unwrap();
-        //                 if *n_sens {
-        //                     nv.str_sens(sens_symb, rc_symb).to_string()
-        //                 } else {
-        //                     nv.str_rc(sens_symb, rc_symb).to_string()
-        //                 }
-        //             } else {
-        //                 nv.str_sens(sens_symb, rc_symb).to_string()
-        //             };
-        //             format!("{val} ({})", ni.index())
-        //         },
-        //         |_, v| v.to_string(),
-        //     );
-        //     println!(
-        //         "{}",
-        //         dot_graph(
-        //             &g,
-        //             &new_way.into_iter().map(|(id, _)| id.clone()).collect(),
-        //             "A",
-        //             "red",
-        //             true,
-        //         )
-        //     );
-        // }
-
-        let res_str: Vec<String> = ways
-            .iter()
-            .map(|way| {
-                let s: Vec<String> = way
+        let mut ranges: Vec<_> = phases
+            .par_chunks(100)
+            .flat_map(|chunks| {
+                let mut bam = rust_htslib::bam::IndexedReader::from_path(bam_path).unwrap();
+                chunks
+                    .to_vec()
                     .iter()
-                    .map(|(node_id, sens)| {
-                        let bp = graph.node_weight(*node_id).unwrap();
-                        match sens {
-                            true => bp.str_sens(sens_symb, rc_symb),
-                            false => bp.str_rc(sens_symb, rc_symb),
-                        }
-                    })
-                    .collect();
-                s.join("")
+                    .map(|p| (p.range(&mut bam), p.mean_vaf(), p.data.len()))
+                    .collect::<Vec<_>>()
             })
+            .filter(|(r, _, _)| r.is_ok())
+            .map(|(r, v, n)| (r.unwrap(), v, n))
+            .map(|((min, min_cov, max_cov, max), v, n)| (min..=max, min_cov..=max_cov, v, n))
             .collect();
 
-        res_str
-            .iter()
-            .enumerate()
-            .for_each(|(i, s)| println!("{}\t{s}", i + 1));
-
-        let all_bp: Vec<BreakPoint> = graph.node_weights().map(|n| n.clone()).collect();
-        println!("{} unique breakpoints considered.", all_bp.len());
-
-        let nodeids_bp: Vec<(NodeIndex, BreakPoint)> =
-            graph.node_indices().zip(all_bp.into_iter()).collect();
-
-        // monallellic way ?
-
-        Ok(())
-    }
-
-    #[test]
-    fn test_graph() -> Result<()> {
-        let dir = "./data_test";
-
-        // Load from fasta in dir.
-        let genome = Genome::from_contigs_sequences(dir)?;
-        let mut genomic_graph = GenomicGraph::from_genome(&genome);
-        let dot = genomic_graph.dot_graph();
-        println!("{dot}");
+        ranges.sort_by(|(a, _, _, _), (b, _, _, _)| a.start().cmp(&b.start()));
 
-        let ways = genomic_graph.ways((true, "chr7", 0), (true, "chr7", i32::MAX));
-        for (i, way) in ways.iter().enumerate() {
-            let s = way
-                .iter()
-                .map(|(_, _, _, s)| s.to_string())
-                .collect::<Vec<String>>()
-                .join("");
-            println!("{}\t{s}", i + 1);
+        for (_i, (range, _cov, vaf, n)) in ranges.iter().enumerate() {
+            if *n < 2 {
+                continue;
+            }
+            let line = vec![
+                "chr1".to_string(),
+                range.start().to_string(),
+                range.end().to_string(),
+                format!(
+                    "{} {} {}",
+                    (range.end() - range.start()).to_formatted_string(&format),
+                    n,
+                    vaf
+                ),
+            ]
+            .join("\t");
+            println!("{line}");
         }
         Ok(())
     }

+ 209 - 0
src/phase.rs

@@ -0,0 +1,209 @@
+use anyhow::{anyhow, Ok, Result};
+use rust_htslib::bam::IndexedReader;
+use std::{cmp::Ordering, collections::HashSet};
+
+#[derive(Debug, Clone)]
+pub struct HeteroVar {
+    pub chr: String,
+    pub position: i32,
+    pub base: u8,
+    pub vaf: f64,
+    pub reads: HashSet<String>,
+}
+
+impl HeteroVar {
+    pub fn new(
+        bam: &mut IndexedReader,
+        chr: &str,
+        position: i32,
+        reference: u8,
+        alternative: u8,
+    ) -> Result<(HeteroVar, HeteroVar)> {
+        let rec_base = if let std::result::Result::Ok(rb) =
+            pileup::qnames_at_base(bam, chr, position, false)
+        {
+            rb
+        } else {
+            return Err(anyhow!("Error while reading BAM file."));
+        };
+        let depth = rec_base.len() as i32;
+        if depth == 0 {
+            return Err(anyhow!("No records"));
+        }
+        let mut a = (reference, Vec::new());
+        let mut b = (alternative, Vec::new());
+        for (record, base) in rec_base.into_iter() {
+            if base == reference {
+                a.1.push(record.clone());
+            } else if base == alternative {
+                b.1.push(record.clone());
+            }
+        }
+
+        let res: Vec<HeteroVar> = vec![a, b]
+            .into_iter()
+            .enumerate()
+            .filter(|(_, (_, rec))| rec.len() > 0)
+            .map(|(_, (base, records))| {
+                let mut records_ids: HashSet<String> = HashSet::new();
+                records_ids.extend(records.into_iter());
+                HeteroVar {
+                    chr: chr.to_string(),
+                    position,
+                    base,
+                    vaf: records_ids.len() as f64 / depth as f64,
+                    reads: records_ids,
+                }
+            })
+            .collect();
+
+        if res.len() == 2 {
+            let a = res.get(0).unwrap().to_owned();
+            let b = res.get(1).unwrap().to_owned();
+            return Ok((a, b));
+        } else {
+            return Err(anyhow!("Not enough reads"));
+        }
+    }
+}
+impl PartialEq for HeteroVar {
+    fn eq(&self, other: &Self) -> bool {
+        self.reads == other.reads
+            && self.chr == other.chr
+            && self.position == other.position
+            && self.base == other.base
+    }
+}
+
+#[derive(Debug, Clone)]
+pub struct Phase {
+    pub data: Vec<HeteroVar>,
+    pub reads: HashSet<String>,
+}
+
+impl Phase {
+    pub fn new(hetero_var: &HeteroVar) -> Self {
+        Self {
+            data: vec![hetero_var.clone()],
+            reads: hetero_var.reads.clone(),
+        }
+    }
+    pub fn try_merge(&mut self, hetero_var: &HeteroVar, min_records: usize) -> bool {
+        if hetero_var
+            .reads
+            .iter()
+            .filter(|r| self.reads.contains(*r))
+            .count()
+            >= min_records
+        {
+            self.reads.extend(hetero_var.reads.clone().into_iter());
+            self.data.push(hetero_var.clone());
+            self.data.sort_by(|a, b| a.position.cmp(&b.position));
+            true
+        } else {
+            false
+        }
+    }
+
+    pub fn try_merge_phase(&mut self, phase: &Phase, min_records: usize) -> bool {
+        if phase
+            .reads
+            .iter()
+            .filter(|r| self.reads.contains(*r))
+            .count()
+            >= min_records
+        {
+            self.reads.extend(phase.reads.clone().into_iter());
+            self.data.extend(phase.data.clone().into_iter());
+            self.data.sort_by(|a, b| a.position.cmp(&b.position));
+            true
+        } else {
+            false
+        }
+    }
+
+    pub fn range(&self, bam: &mut IndexedReader) -> Result<(i64, i64, i64, i64)> {
+        let mut phase = self.clone();
+        phase.sort_dedup();
+        let data = &self.data;
+
+        let left = data.first().unwrap();
+        let right = data.last().unwrap();
+
+        let left_records = pileup::records_at_base(bam, &left.chr, left.position, true)?;
+        let right_records = pileup::records_at_base(bam, &right.chr, right.position, true)?;
+
+        let left_starts: Vec<_> = left_records
+            .iter()
+            .filter(|(_, b)| *b == left.base)
+            .map(|(r, _)| r.pos())
+            .collect();
+        let right_ends: Vec<_> = right_records
+            .iter()
+            .filter(|(_, b)| *b == right.base)
+            .map(|(r, _)| r.pos() + r.seq_len() as i64)
+            .collect();
+
+        let min = left_starts.iter().min();
+        let min_cov = left_starts.iter().max();
+        let max_cov = right_ends.iter().min();
+        let max = right_ends.iter().max();
+
+        if min.is_some() && min_cov.is_some() && max.is_some() && max_cov.is_some() {
+            Ok((
+                *min.unwrap(),
+                *min_cov.unwrap(),
+                *max_cov.unwrap(),
+                *max.unwrap(),
+            ))
+        } else {
+            Err(anyhow!("problem"))
+        }
+    }
+
+    pub fn mean_vaf(&self) -> f64 {
+        let vafs: Vec<_> = self.data.iter().map(|s| s.vaf).collect();
+        vafs.iter().sum::<f64>() / vafs.len() as f64
+    }
+
+    pub fn sort_dedup(&mut self) {
+        self.data.sort_by(|a, b| a.position.cmp(&b.position));
+        self.data
+            .dedup_by(|a, b| a.position == b.position && a.chr == b.chr && a.base == b.base);
+    }
+
+    pub fn bed_string(&self, bam: &mut IndexedReader) -> Result<String> {
+        let first = self.data.first().unwrap();
+        let (min, min_cov, max_cov, max) = self.range(bam)?;
+        Ok(vec![
+            first.chr.to_string(),
+            min_cov.to_string(),
+            max_cov.to_string(),
+            self.mean_vaf().to_string(),
+        ]
+        .join("\t"))
+    }
+}
+
+impl Ord for Phase {
+    fn cmp(&self, other: &Self) -> Ordering {
+        let s = self.data.first().unwrap();
+        let other = other.data.first().unwrap();
+        s.position.cmp(&other.position)
+    }
+}
+
+impl PartialOrd for Phase {
+    fn partial_cmp(&self, other: &Self) -> Option<Ordering> {
+        Some(self.cmp(other))
+    }
+}
+
+impl PartialEq for Phase {
+    fn eq(&self, other: &Self) -> bool {
+        self.reads == other.reads
+        // self.data.iter().filter(|s| other.data.contains(s)).count() == other.data.len()
+    }
+}
+
+impl Eq for Phase {}

+ 3847 - 0
test.bed

@@ -0,0 +1,3847 @@
+chr1	1	200383	200_382 1313 0.4426115876549664
+chr1	86938	200383	113_445 177 0.433119400211477
+chr1	177571	199195	21_624 4 0.3832251726601572
+chr1	206555	240659	34_104 22 0.3804312103771226
+chr1	236337	254135	17_798 10 0.40003885003885004
+chr1	237341	251521	14_180 10 0.46149961149961144
+chr1	272461	298485	26_024 5 0.6113500663423264
+chr1	272465	296733	24_268 5 0.36635891198584697
+chr1	290819	320503	29_684 82 0.4420519486554373
+chr1	293561	320503	26_942 46 0.44256947454214923
+chr1	326495	636117	309_622 999 0.48094823590744484
+chr1	328679	636117	307_438 2821 0.4803953773810678
+chr1	392888	407346	14_458 3 0.2818181818181818
+chr1	458535	636117	177_582 632 0.48360325828437084
+chr1	495859	636117	140_258 527 0.4793847597809241
+chr1	562755	636117	73_362 437 0.5020649405080255
+chr1	581137	590475	9_338 8 0.43154761904761907
+chr1	582750	590475	7_725 7 0.2688311688311688
+chr1	605185	636117	30_932 50 0.465347507564899
+chr1	633224	787204	153_980 1353 0.4834381948434613
+chr1	772451	895935	123_484 512 0.4906014011502211
+chr1	864842	895935	31_093 18 0.42125644417311076
+chr1	872997	1056466	183_469 766 0.497021649940682
+chr1	965132	1056466	91_334 549 0.5022838319281988
+chr1	1050850	1058641	7_791 5 0.35174603174603175
+chr1	1052968	1095208	42_240 11 0.5994394494394494
+chr1	1058199	1073092	14_893 3 0.35073260073260076
+chr1	1058359	1099370	41_011 3 0.47341269841269845
+chr1	1077033	1111576	34_543 9 0.5400641025641026
+chr1	1098868	1165490	66_622 51 0.5398018970053511
+chr1	1107256	1128877	21_621 3 0.28086419753086417
+chr1	1114629	1143230	28_601 6 0.40029761904761907
+chr1	1151240	1229936	78_696 282 0.4820201174375826
+chr1	1222615	1247527	24_912 6 0.3956788710907704
+chr1	1227236	1255418	28_182 5 0.5634782608695652
+chr1	1242589	1321999	79_410 152 0.4928549527550466
+chr1	1314157	1450718	136_561 544 0.4836706689779118
+chr1	1447227	1530132	82_905 339 0.493241545830604
+chr1	1515955	1587350	71_395 1586 0.43205102341257495
+chr1	1592490	1616670	24_180 3 0.3972038558293818
+chr1	1605449	1650089	44_640 21 0.5078007518796992
+chr1	1613723	1650089	36_366 20 0.4802083333333334
+chr1	1641049	1941238	300_189 2712 0.47830406585794305
+chr1	1859432	1941238	81_806 183 0.48148991455949747
+chr1	1965660	1995766	30_106 3 0.5580808080808081
+chr1	1979228	2010569	31_341 4 0.4751225490196078
+chr1	1989407	2020136	30_729 9 0.4693355119825708
+chr1	2076288	2240932	164_644 400 0.4157294097686339
+chr1	2091618	2240932	149_314 1249 0.37032352051554646
+chr1	2172613	2189564	16_951 3 0.2888888888888889
+chr1	2242076	2283375	41_299 70 0.43745735033729743
+chr1	2314645	2507818	193_173 1763 0.482648045669262
+chr1	2481230	2495482	14_252 3 0.3052503052503052
+chr1	2510442	2563019	52_577 209 0.4795375238898163
+chr1	2557199	2576808	19_609 6 0.5394736842105262
+chr1	2561932	2579148	17_216 6 0.45175438596491224
+chr1	2563497	3044979	481_482 6383 0.4826201082442206
+chr1	2967197	3044979	77_782 211 0.4732931445406732
+chr1	3017841	3250664	232_823 589 0.4771761210934668
+chr1	3034745	3250664	215_919 565 0.47386419195971924
+chr1	3069244	3250664	181_420 1975 0.46130528875987703
+chr1	3229588	3243390	13_802 3 0.48677248677248675
+chr1	3235685	3307751	72_066 216 0.5029102848727706
+chr1	3287080	3312206	25_126 5 0.4179767600820233
+chr1	3303004	3344358	41_354 4 0.5969469127363864
+chr1	3338376	3350275	11_899 10 0.5252380952380952
+chr1	3346649	3383394	36_745 15 0.5039849624060151
+chr1	3363979	3552997	189_018 1354 0.4898394286653446
+chr1	3451160	3552997	101_837 240 0.5011978552041867
+chr1	3533783	3556041	22_258 5 0.37035087719298243
+chr1	3554171	3704011	149_840 1391 0.4735567785733984
+chr1	3636118	3855946	219_828 1100 0.486436210556975
+chr1	3832901	3939366	106_465 712 0.4929462950683235
+chr1	3920676	4185749	265_073 1777 0.48635923580905505
+chr1	4163236	4186510	23_274 18 0.509057781361703
+chr1	4166196	4184344	18_148 11 0.476087266862668
+chr1	4175699	4188194	12_495 7 0.48571428571428565
+chr1	4188391	4356238	167_847 892 0.4935904221669547
+chr1	4311540	4325065	13_525 4 0.2976190476190476
+chr1	4351981	4396641	44_660 167 0.49075205874022093
+chr1	4409032	4716544	307_512 1256 0.48894311669839235
+chr1	4537654	4554345	16_691 6 0.39747474747474754
+chr1	4550919	4573010	22_091 11 0.34213201472253835
+chr1	4573479	4776725	203_246 889 0.4829533207266422
+chr1	4769775	4840364	70_589 650 0.48973216091645555
+chr1	4885202	5075605	190_403 1065 0.48831291823255213
+chr1	5051990	5087364	35_374 10 0.4220094973153259
+chr1	5059275	5088239	28_964 14 0.49261770657226206
+chr1	5076799	5216194	139_395 845 0.48847721620980683
+chr1	5083876	5129494	45_618 64 0.4606645275274356
+chr1	5213787	5297157	83_370 543 0.5091913118671765
+chr1	5293811	5635163	341_352 2735 0.4880908353350059
+chr1	5408765	5635163	226_398 1310 0.4794167619985906
+chr1	5573673	5635163	61_490 88 0.4769070689426877
+chr1	5664432	5681392	16_960 9 0.2998663373663374
+chr1	5665317	5756257	90_940 287 0.5005485020033353
+chr1	5731197	5758922	27_725 8 0.4963725744381237
+chr1	5753164	5775622	22_458 4 0.49523809523809526
+chr1	5753373	5957537	204_164 920 0.4874233024239376
+chr1	5760239	5781905	21_666 3 0.5208333333333334
+chr1	5943645	5990967	47_322 80 0.45874951759873606
+chr1	5986982	6013828	26_846 4 0.5270499108734402
+chr1	5989523	6013169	23_646 4 0.46158645276292337
+chr1	6024867	6196233	171_366 785 0.48832958580634234
+chr1	6205727	6232530	26_803 3 0.3632759784075574
+chr1	6209174	6322986	113_812 145 0.49435548420121916
+chr1	6317097	6340493	23_396 4 0.5
+chr1	6332686	6379356	46_670 14 0.6174409460753998
+chr1	6334859	6381103	46_244 14 0.3641752458559181
+chr1	6377026	6401643	24_617 10 0.5882352941176471
+chr1	6396007	6435481	39_474 15 0.5086415680874333
+chr1	6399220	6416094	16_874 5 0.3611743158801982
+chr1	6411840	6440377	28_537 9 0.46223031754432725
+chr1	6427339	6451411	24_072 5 0.5313131313131313
+chr1	6429921	6487015	57_094 256 0.4621533975270553
+chr1	6486899	6630442	143_543 794 0.49547142299924113
+chr1	6613243	6654655	41_412 35 0.4717100348911494
+chr1	6640287	6744941	104_654 312 0.48838465254331925
+chr1	6740171	6812481	72_310 161 0.5080096352760803
+chr1	6783248	6843006	59_758 144 0.48090836789977004
+chr1	6815360	6838448	23_088 8 0.4788587229763701
+chr1	6832441	7123474	291_033 2229 0.4908969464648766
+chr1	7137787	7493723	355_936 3270 0.4912649734746881
+chr1	7454740	7463536	8_796 3 0.436026936026936
+chr1	7454740	7493723	38_983 47 0.45243943567391376
+chr1	7484349	7665986	181_637 863 0.4815093582242295
+chr1	7572617	7665986	93_369 260 0.49280950502911336
+chr1	7656896	7727387	70_491 81 0.47485421056007804
+chr1	7662360	7727387	65_027 82 0.5381205721516681
+chr1	7718731	7787668	68_937 254 0.5013805768764064
+chr1	7722264	7787668	65_404 261 0.5026518922810012
+chr1	7794679	7820978	26_299 3 0.35569315445476435
+chr1	7800065	7829957	29_892 3 0.5849329205366357
+chr1	7813827	7997107	183_280 950 0.4869219582310037
+chr1	7947121	7982591	35_470 21 0.4997699066113388
+chr1	7982530	8096975	114_445 289 0.4790176928340594
+chr1	7992313	8023100	30_787 21 0.4644021633517432
+chr1	8090694	8158231	67_537 46 0.5040032880173105
+chr1	8137685	8154631	16_946 3 0.3318054494525083
+chr1	8148538	8431144	282_606 479 0.5051110749549951
+chr1	8420976	8564940	143_964 783 0.5057521057457505
+chr1	8551430	8667324	115_894 868 0.5019317201654923
+chr1	8657878	8675947	18_069 3 0.45299145299145294
+chr1	8669323	8732742	63_419 58 0.4820240071808714
+chr1	8670429	8734274	63_845 121 0.4902713424555958
+chr1	8746612	8803371	56_759 57 0.4655348898883304
+chr1	8747830	8803371	55_541 97 0.49902642839271105
+chr1	8804959	9074805	269_846 1662 0.4847018105207367
+chr1	9039596	9229517	189_921 542 0.49498664136220055
+chr1	9075365	9082734	7_369 4 0.3573717948717948
+chr1	9211824	9382902	171_078 772 0.5148135263526711
+chr1	9361212	9401822	40_610 19 0.36151220453616467
+chr1	9377689	9419066	41_377 10 0.6774928774928776
+chr1	9447409	9541675	94_266 247 0.5034739994189135
+chr1	9538722	9607403	68_681 77 0.5159264774840456
+chr1	9612578	9675794	63_216 18 0.48535548865915934
+chr1	9657694	9826937	169_243 183 0.5065840164572853
+chr1	9669926	9826937	157_011 434 0.49314388041124774
+chr1	9768507	9789536	21_029 3 0.3239962651727358
+chr1	9781078	9826937	45_859 27 0.4839646047482187
+chr1	9796803	9841330	44_527 8 0.4967636646190462
+chr1	9867831	9954258	86_427 129 0.4850451439119725
+chr1	9952966	10065308	112_342 374 0.49628876672019834
+chr1	10056307	10087956	31_649 5 0.5346702317290553
+chr1	10057645	10085400	27_755 5 0.43957219251336904
+chr1	10078762	10144434	65_672 235 0.5007802322138993
+chr1	10140180	10317128	176_948 316 0.501099107165955
+chr1	10147318	10163033	15_715 3 0.34415584415584416
+chr1	10163742	10241324	77_582 73 0.4899148311292593
+chr1	10232076	10317128	85_052 78 0.49207672743072406
+chr1	10235887	10304235	68_348 118 0.49153078033657116
+chr1	10279455	10598938	319_483 1542 0.49015717100980116
+chr1	10605272	10630072	24_800 4 0.5456349206349206
+chr1	10611186	10630084	18_898 3 0.46296296296296297
+chr1	10623881	10661265	37_384 5 0.48877501560224673
+chr1	10636824	10682004	45_180 12 0.5600875858635959
+chr1	10679132	10707019	27_887 3 0.586530264279625
+chr1	10751484	10778289	26_805 4 0.5213690476190476
+chr1	10752610	10778086	25_476 4 0.4786309523809523
+chr1	10785318	10816149	30_831 3 0.5066293183940243
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+ 1948 - 0
test_1.bed

@@ -0,0 +1,1948 @@
+
+running 1 test
+test tests::phasing has been running for over 60 seconds
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+chr1	248090493	248378444	287_951 2482 0.48370693458454395
+test tests::phasing ... ok
+
+test result: ok. 1 passed; 0 failed; 0 ignored; 0 measured; 2 filtered out; finished in 876.09s
+

+ 8975 - 0
test_2.bed

@@ -0,0 +1,8975 @@
+
+running 1 test
+test tests::phasing has been running for over 60 seconds
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+chr1	247823388	247907149	83_761 392 0.4633615634179477
+chr1	247823641	248004265	180_624 1433 0.4781607635883423
+chr1	247823641	247907149	83_508 392 0.4639284568419839
+chr1	247884879	248021404	136_525 916 0.49048402052512635
+chr1	247884879	248004265	119_386 369 0.4961439074861766
+chr1	247965687	248004265	38_578 154 0.47300473272589394
+chr1	247992046	248021404	29_358 9 0.43091168091168097
+chr1	248088661	248290057	201_396 787 0.4947345433170595
+chr1	248090493	248378444	287_951 2615 0.48945415273271975
+chr1	248090493	248290057	199_564 775 0.4876357334596677
+chr1	248183091	248290057	106_966 188 0.5109690835337638
+chr1	248239110	248378444	139_334 774 0.4655712462108858
+chr1	248335762	248349750	13_988 4 0.375
+test tests::phasing ... ok
+
+test result: ok. 1 passed; 0 failed; 0 ignored; 0 measured; 2 filtered out; finished in 35134.30s
+

+ 4208 - 0
test_3.bed

@@ -0,0 +1,4208 @@
+
+chr1	1	200383	200_382 1313 0.4426115876549664
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+ 3617 - 0
test_4.bed

@@ -0,0 +1,3617 @@
+
+running 1 test
+test tests::phasing has been running for over 60 seconds
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+chr1	247163747	247203670	39_923 15 0.48409831292589917
+chr1	247185457	247205635	20_178 12 0.49164196116782327
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+chr1	247221810	247363442	141_632 2048 0.4847061730050511
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+chr1	247823388	248021404	198_016 1973 0.480648290278334
+chr1	248090493	248378444	287_951 2984 0.4871870654948597
+test tests::phasing ... ok
+
+test result: ok. 1 passed; 0 failed; 0 ignored; 0 measured; 2 filtered out; finished in 266.33s
+

+ 1852 - 0
test_5.bed

@@ -0,0 +1,1852 @@
+
+running 1 test
+test tests::phasing has been running for over 60 seconds
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+chr1	219574586	219870794	296_208 2203 0.48018010563855335
+chr1	220131511	220145580	14_069 4 0.29657738095238095
+chr1	220977569	220987576	10_007 8 0.3641462703962704
+chr1	221984507	222099372	114_865 196 0.47552091310202765
+chr1	222082483	222335175	252_692 1503 0.48338739986688484
+chr1	222330684	222568203	237_519 1397 0.48855922061395496
+chr1	222591387	222646397	55_010 6 0.5028414511600164
+chr1	222610603	222635765	25_162 3 0.4529637142213207
+chr1	222628907	222669532	40_625 5 0.5754008677607998
+chr1	222666877	222695714	28_837 16 0.49256048387096785
+chr1	222689903	222745042	55_139 311 0.4576587561007811
+chr1	222720784	222745694	24_910 3 0.5020703933747411
+chr1	222740376	222755527	15_151 6 0.4078282828282827
+chr1	223452445	223492286	39_841 5 0.51
+chr1	223489710	223517734	28_024 14 0.5
+chr1	223669845	223819393	149_548 852 0.4842491728324357
+chr1	223809509	223846786	37_277 12 0.61379826719739
+chr1	223815382	223842109	26_727 12 0.35612694647782367
+chr1	223839457	223911939	72_482 282 0.4957084264016315
+chr1	223938072	224314067	375_995 2502 0.49174063327978895
+chr1	224307987	224599882	291_895 1769 0.4876676688389298
+chr1	224591661	224653234	61_573 244 0.4945162133524326
+chr1	224655369	224679029	23_660 20 0.5
+chr1	224670906	224721152	50_246 8 0.47893318654693
+chr1	224778292	224810687	32_395 3 0.4895061728395061
+chr1	224834798	224853394	18_596 6 0.6421957671957673
+chr1	224847157	224882213	35_056 5 0.5548309178743961
+chr1	224862888	224895982	33_094 5 0.42777777777777776
+chr1	224887998	225113022	225_024 880 0.4831440039714013
+chr1	225118846	225692970	574_124 3317 0.4873918250474345
+chr1	225699689	226004163	304_474 1246 0.48388795089686115
+chr1	226205348	226238183	32_835 3 0.5387667887667887
+chr1	226206056	226235582	29_526 3 0.40781440781440775
+chr1	226285899	226320174	34_275 3 0.4666332497911445
+chr1	226288207	226320032	31_825 3 0.4999498746867168
+chr1	226340852	226367618	26_766 8 0.49375
+chr1	226435818	226447741	11_923 5 0.5
+chr1	226492516	226707689	215_173 816 0.4872789591015799
+chr1	226703739	226717683	13_944 5 0.5111111111111111
+chr1	226706403	226722802	16_399 5 0.48888888888888893
+chr1	226716040	226844807	128_767 655 0.4941806214375059
+chr1	226842263	227018243	175_980 602 0.47784187825492713
+chr1	227011133	227138598	127_465 456 0.4810693238157994
+chr1	227108836	227645138	536_302 2665 0.4866505806088247
+chr1	227661529	227692616	31_087 4 0.48863636363636365
+chr1	227662418	227692782	30_364 4 0.471969696969697
+chr1	227692539	227726598	34_059 3 0.4966329966329966
+chr1	227693510	227726242	32_732 4 0.47310606060606064
+chr1	227737074	228176501	439_427 1288 0.4901711902813235
+chr1	228173399	228266613	93_214 801 0.4890648428434566
+chr1	228262596	228362478	99_882 421 0.4940117778915553
+chr1	228356823	228404300	47_477 115 0.4755987911404041
+chr1	228455456	228721242	265_786 1156 0.4866344342142929
+chr1	228750570	228973373	222_803 1646 0.48794253062550386
+chr1	229152641	229200424	47_783 189 0.4536735971454125
+chr1	229236745	229395504	158_759 1031 0.47722584315025807
+chr1	229409340	229440314	30_974 4 0.484375
+chr1	229442466	229504269	61_803 303 0.49508025028371416
+chr1	229770360	230036384	266_024 2194 0.4877266176722985
+chr1	230030556	230205402	174_846 1118 0.48812389037859044
+chr1	230246820	230285913	39_093 32 0.48088769425654454
+chr1	230274501	230429923	155_422 1060 0.48840669061417874
+chr1	230417860	230526205	108_345 255 0.49268491061519176
+chr1	230533309	230574302	40_993 73 0.4824123877061501
+chr1	230633390	230652581	19_191 7 0.40216086434573833
+chr1	230749467	230802519	53_052 9 0.5067679301374953
+chr1	231070907	231113228	42_321 18 0.503339947089947
+chr1	231092536	231174890	82_354 178 0.4941710477625649
+chr1	231278013	231453163	175_150 1666 0.49584186628598037
+chr1	231479779	231532969	53_190 41 0.5200884324632721
+chr1	231544883	231580372	35_489 21 0.4791988875322209
+chr1	231570028	232031671	461_643 2854 0.48572157772057073
+chr1	232034266	232050836	16_570 6 0.5
+chr1	232048465	232175361	126_896 604 0.48805723412611046
+chr1	232193150	232328679	135_529 491 0.4853517837777625
+chr1	232513159	232550321	37_162 101 0.4964698875902626
+chr1	232544182	232593379	49_197 8 0.45975140056022407
+chr1	232545137	232580233	35_096 7 0.5364745898359343
+chr1	232585891	232655418	69_527 127 0.5015875513788336
+chr1	232612072	232697847	85_775 62 0.46576365099188555
+chr1	232716831	232889763	172_932 1340 0.49165296096361305
+chr1	232949809	232978666	28_857 14 0.5439097744360903
+chr1	232960978	233073990	113_012 395 0.47976991151143145
+chr1	233077540	233260453	182_913 1001 0.48800102261708483
+chr1	233256884	233545387	288_503 2218 0.4910193168338279
+chr1	233548912	233576913	28_001 5 0.5104761904761904
+chr1	233567592	233609609	42_017 81 0.48913207458545876
+chr1	233605409	233630870	25_461 15 0.47932263814616755
+chr1	233633037	234467919	834_882 6172 0.4862716872318554
+chr1	234579335	234604267	24_932 3 0.6285266457680251
+chr1	234638214	234697111	58_897 9 0.501010101010101
+chr1	234749469	234844004	94_535 275 0.49169434092708475
+chr1	234864761	234897031	32_270 12 0.46341851004322393
+chr1	234907482	235105376	197_894 1302 0.484403891159592
+chr1	235095744	235175953	80_209 53 0.48263238021771343
+chr1	235163927	235263656	99_729 396 0.48707432226671493
+chr1	235269034	235583839	314_805 2888 0.47371743896776414
+chr1	235587907	235616899	28_992 3 0.425
+chr1	235593802	236008456	414_654 5223 0.4918843053448684
+chr1	236000471	236546655	546_184 5545 0.4851172688826834
+chr1	236556682	236689186	132_504 761 0.48767937328137034
+chr1	236673709	237337200	663_491 12824 0.4848276008702368
+chr1	237319309	237357818	38_509 13 0.503651085756349
+chr1	237330759	237362158	31_399 11 0.4214945660400206
+chr1	237397758	237430011	32_253 3 0.4898989898989899
+chr1	237399251	237431258	32_007 3 0.5
+chr1	237436337	237536797	100_460 425 0.48937961394073
+chr1	237527881	238007278	479_397 4247 0.4839437867648735
+chr1	237960741	237989217	28_476 4 0.5428571428571428
+chr1	238005353	238070815	65_462 241 0.48441890968579826
+chr1	238085236	238145195	59_959 84 0.49350261759822295
+chr1	238124093	238147310	23_217 3 0.5303643724696356
+chr1	238160515	238319376	158_861 627 0.49096873063732366
+chr1	238305992	238372072	66_080 172 0.4871578108871894
+chr1	238720754	239146839	426_085 3027 0.48257820294430137
+chr1	239137063	239375329	238_266 694 0.4830865714216523
+chr1	239424128	239483846	59_718 615 0.4825085389326063
+chr1	239483643	239514426	30_783 29 0.512021072796935
+chr1	239510545	239542091	31_546 10 0.5890857233581692
+chr1	239514898	239544375	29_477 10 0.4032219689495231
+chr1	239535535	239680659	145_124 840 0.4768801768768868
+chr1	239673887	239751996	78_109 477 0.485020422830673
+chr1	239741524	239768752	27_228 8 0.4603578693109585
+chr1	239769593	239881237	111_644 416 0.45659681558276605
+chr1	239778118	239798477	20_359 3 0.47908496732026146
+chr1	239879822	240020839	141_017 587 0.48821820148062045
+chr1	240014552	240056248	41_696 133 0.4603995936290154
+chr1	240060806	240145789	84_983 339 0.49390498963445023
+chr1	240267860	240280436	12_576 4 0.9159919028340081
+chr1	240319545	240344144	24_599 5 0.5594736842105263
+chr1	240338700	240456727	118_027 372 0.4809136748775814
+chr1	240452988	240473793	20_805 5 0.5076923076923077
+chr1	240563234	240641519	78_285 149 0.46595857297022014
+chr1	240635129	240661822	26_693 13 0.5213675213675214
+chr1	240651768	240787109	135_341 529 0.5008899673199353
+chr1	240706361	240724132	17_771 7 0.3833800186741363
+chr1	240783233	240811258	28_025 85 0.4817453513417977
+chr1	240804283	240828710	24_427 22 0.4745143745143745
+chr1	240824206	240881572	57_366 201 0.47702914492829485
+chr1	240876226	244060590	3_184_364 6278 0.4862225119248896
+chr1	240941503	241032663	91_160 161 0.4892243449831891
+chr1	241027927	241563754	535_827 4793 0.4852078512739677
+chr1	241564383	241585895	21_512 23 0.4989039093898429
+chr1	241588672	241648645	59_973 230 0.4853380120101208
+chr1	241633781	241734678	100_897 451 0.4868759347826469
+chr1	241747795	241841825	94_030 626 0.4896722533509613
+chr1	241840250	242215498	375_248 2549 0.4868707022932888
+chr1	242210210	242439408	229_198 1885 0.4798460724383581
+chr1	242449973	242559104	109_131 96 0.47680049596097374
+chr1	242450559	242483900	33_341 6 0.42942610691089395
+chr1	242547158	242564913	17_755 3 0.40598290598290604
+chr1	242562102	242758291	196_189 569 0.4839466158989973
+chr1	242851890	242904702	52_812 85 0.5002994023296217
+chr1	242901475	242946469	44_994 42 0.47208656562040024
+chr1	242926474	243120913	194_439 747 0.4836097525744679
+chr1	243116625	243351203	234_578 1098 0.4829861886632453
+chr1	243155979	243180170	24_191 7 0.42280966790283564
+chr1	243349286	243541779	192_493 857 0.48201722387767526
+chr1	243531805	243609395	77_590 303 0.48930355377119333
+chr1	244058913	244557747	498_834 3240 0.4866718170893676
+chr1	244555904	244577830	21_926 9 0.4957671957671958
+chr1	245237857	245397476	159_619 1062 0.4829134274869235
+chr1	245386985	245653706	266_721 3231 0.4807750497317152
+chr1	245634881	246140492	505_611 4422 0.4832636633441097
+chr1	246125610	246269671	144_061 939 0.40032537106135735
+chr1	246293005	246638427	345_422 5055 0.4815632111549514
+chr1	247123109	247156787	33_678 4 0.4720238095238095
+chr1	247162608	247188742	26_134 3 0.443988603988604
+chr1	247163747	247203670	39_923 15 0.48409831292589917
+chr1	247185457	247222418	36_961 69 0.4866074800625095
+chr1	247433436	247711716	278_280 1740 0.4819352883741606
+chr1	247751486	247827163	75_677 813 0.4901269932535025
+chr1	247823641	248021404	197_763 1900 0.4720850182718802
+chr1	248090493	248378444	287_951 2482 0.48370693458454395
+test tests::phasing ... ok
+
+test result: ok. 1 passed; 0 failed; 0 ignored; 0 measured; 2 filtered out; finished in 1225.37s
+

+ 3 - 0
test_6.bed

@@ -0,0 +1,3 @@
+
+running 1 test
+test tests::phasing has been running for over 60 seconds

BIN
test_chr1.bytes.gz


Деякі файли не було показано, через те що забагато файлів було змінено