flowchart TD
    A["Variants from first pass 1,055,434"] --> B{Constit BAM pileup 
    depth at the variant
    position}
    B --> | < 6| C["Low Constit depth 718,656 (68%)"]
    B --> D{Variant seen 
    at least one time 
    in the pileup}
    D --> |yes| E["Constit 128,065 (12%)"]
    D --> |no| F{Take -20 +20 
    reference sequence
    check diversity }
    F --> |low entropy < 1.8| H["Low diversity 183,528 (17%)"]
    F --> I["Somatic 24,331 (2,3% 0,55%)"]
