flowchart TD
    Z["Variants from VCFs constit: 3,504,804"]--> ZZ["Into constit registry"] --> B
    A["Variants from VCFs bulk: 4,458,549"] -->|First pass| B{Variant is in 
    constit registry ?}
    B --> |yes| C{Constit depth < 6}
    B --> |no| I["Variants for second pass 1,055,434 (24%)"]
    C --> |yes| D["Low constit depth: 1,070,325 (24%)"]
    C --> |no| E{VAF}
    E --> |100%| F["Constit 2,329,315 (52%)"]
    E --> G{Chi2 AF pval}
    G --> |< 0.01| H["LOH 3,474 (0,1%)"]
    G --> F