flowchart TD A["Variants from first pass 1,055,434"] --> B{Constit BAM pileup depth at the variant position} B --> | < 6| C["Low Constit depth 718,656 (68%)"] B --> D{Variant seen at least one time in the pileup} D --> |yes| E["Constit 128,065 (12%)"] D --> |no| F{Take -20 +20 reference sequence check diversity } F --> |low entropy < 1.8| H["Low diversity 183,528 (17%)"] F --> I["Somatic 24,331 (2,3% 0,55%)"]