Thomas 4 роки тому
батько
коміт
ae7da36fc0

BIN
Images/dko_zfp_surv.png


+ 12 - 5
presentation.aux

@@ -133,8 +133,15 @@
 \HyPL@Entry{23<</P(20)>>}
 \@writefile{nav}{\defcounter {refsection}{0}\relax }\@writefile{nav}{\headcommand {\slideentry {4}{0}{13}{24/24}{}{0}}}
 \@writefile{nav}{\defcounter {refsection}{0}\relax }\@writefile{nav}{\headcommand {\beamer@framepages {24}{24}}}
-\@writefile{nav}{\headcommand {\beamer@partpages {1}{24}}}
-\@writefile{nav}{\headcommand {\beamer@subsectionpages {12}{24}}}
-\@writefile{nav}{\headcommand {\beamer@sectionpages {12}{24}}}
-\@writefile{nav}{\headcommand {\beamer@documentpages {24}}}
-\@writefile{nav}{\headcommand {\gdef \inserttotalframenumber {20}}}
+\pbs@newkey{pbs@last@page}{25}
+\HyPL@Entry{24<</P(21)>>}
+\@writefile{nav}{\defcounter {refsection}{0}\relax }\@writefile{nav}{\headcommand {\slideentry {4}{0}{14}{25/25}{}{0}}}
+\@writefile{nav}{\defcounter {refsection}{0}\relax }\@writefile{nav}{\headcommand {\beamer@framepages {25}{25}}}
+\pbs@newkey{pbs@last@page}{26}
+\@writefile{nav}{\defcounter {refsection}{0}\relax }\@writefile{nav}{\headcommand {\slideentry {4}{0}{15}{26/26}{}{0}}}
+\@writefile{nav}{\defcounter {refsection}{0}\relax }\@writefile{nav}{\headcommand {\beamer@framepages {26}{26}}}
+\@writefile{nav}{\headcommand {\beamer@partpages {1}{26}}}
+\@writefile{nav}{\headcommand {\beamer@subsectionpages {12}{26}}}
+\@writefile{nav}{\headcommand {\beamer@sectionpages {12}{26}}}
+\@writefile{nav}{\headcommand {\beamer@documentpages {26}}}
+\@writefile{nav}{\headcommand {\gdef \inserttotalframenumber {21}}}

+ 3 - 3
presentation.blg

@@ -1,5 +1,5 @@
 [0] Config.pm:312> INFO - This is Biber 2.15
 [0] Config.pm:315> INFO - Logfile is 'presentation.blg'
-[48] biber:330> INFO - === mar. juin  1, 2021, 18:03:35
-[56] Biber.pm:415> INFO - Reading 'presentation.bcf'
-[99] Utils.pm:384> WARN - No data sources defined!
+[49] biber:330> INFO - === mer. juin  2, 2021, 18:02:47
+[57] Biber.pm:415> INFO - Reading 'presentation.bcf'
+[100] Utils.pm:384> WARN - No data sources defined!

+ 20 - 20
presentation.fdb_latexmk

@@ -1,16 +1,16 @@
 # Fdb version 3
-["biber presentation"] 1622563414 "presentation.bcf" "presentation.bbl" "presentation" 1622563418
-  "presentation.bcf" 1622563417 101149 070b8d437a9377c293336e898b92955c "xelatex"
+["biber presentation"] 1622649767 "presentation.bcf" "presentation.bbl" "presentation" 1622824651
+  "presentation.bcf" 1622824650 101149 070b8d437a9377c293336e898b92955c "xelatex"
   (generated)
   "presentation.bbl"
   "presentation.blg"
-["xdvipdfmx"] 1622563417 "presentation.xdv" "presentation.pdf" "presentation" 1622563418
-  "presentation.xdv" 1622563417 100344 9e81ae404714b1098ba5fffbf12a046f "xelatex"
+["xdvipdfmx"] 1622824650 "presentation.xdv" "presentation.pdf" "presentation" 1622824651
+  "presentation.xdv" 1622824650 126604 731b136a067c59f47a2bc9217283f8c1 "xelatex"
   (generated)
   "presentation.pdf"
-["xelatex"] 1622563416 "/home/thomas/Documents/Présentations/Translocations_Capture/presentation.tex" "presentation.xdv" "presentation" 1622563418
+["xelatex"] 1622824649 "/home/thomas/Documents/Présentations/Translocations_Capture/presentation.tex" "presentation.xdv" "presentation" 1622824651
   "/etc/texmf/web2c/texmf.cnf" 1610016767 475 c0e671620eb5563b2130f56340a5fde8 ""
-  "/home/thomas/Documents/Présentations/Translocations_Capture/presentation.tex" 1622563414 9819 3966f19b43ef80e74a1351a5c28e218d ""
+  "/home/thomas/Documents/Présentations/Translocations_Capture/presentation.tex" 1622824647 12221 0a37e41b7656b39bc7b9ac8d29622934 ""
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@@ -146,7 +146,6 @@
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   "Images/assemblage.pdf" 1614701719 27648 ed0d81e4fd45d7413d38514055c748af ""
+  "Images/dko_zfp_surv.png" 1622470852 24059 43940d17937b490d8bc759ff91adcc2a ""
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   "Images/gviz_stacked/TRD-TAL1.pdf" 1622119173 13852 8b2d45163dce563758390489db08b32e ""
@@ -289,20 +289,20 @@
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   "Images/translocation_10_14.pdf" 1613992812 2125570 7198b17bf53e357d3c137c7aeb94e30a ""
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-  "presentation.tex" 1622563414 9819 3966f19b43ef80e74a1351a5c28e218d ""
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+  "presentation.tex" 1622824647 12221 0a37e41b7656b39bc7b9ac8d29622934 ""
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   (generated)
-  "presentation.out"
-  "presentation.aux"
-  "presentation.bcf"
-  "presentation.snm"
   "presentation.run.xml"
-  "presentation.xdv"
   "presentation.toc"
-  "presentation.log"
   "presentation.nav"
+  "presentation.out"
+  "presentation.log"
+  "presentation.bcf"
+  "presentation.xdv"
+  "presentation.aux"
+  "presentation.snm"

+ 1 - 2
presentation.fls

@@ -402,8 +402,6 @@ INPUT /usr/share/texlive/texmf-dist/tex/latex/l3packages/l3keys2e/l3keys2e.sty
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 INPUT /usr/share/texlive/texmf-dist/tex/latex/beamer/beamerfontthemeprofessionalfonts.sty
 INPUT /usr/share/texlive/texmf-dist/tex/latex/beamer/beamerfontthemeprofessionalfonts.sty
 INPUT presentation.aux
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 INPUT Images/RAG_ZFP36L2_mech.pdf
 INPUT Images/RAG_ZFP_TREC_ZFP.pdf
 INPUT Images/RSS.pdf
+INPUT Images/dko_zfp_surv.png
 OUTPUT presentation.nav
 OUTPUT presentation.toc
 OUTPUT presentation.snm

+ 75 - 65
presentation.log

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 entering extended mode
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 *geometry* driver: auto-detecting
 *geometry* detected driver: xetex
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 Package hyperref Warning: Height of page (\stockheight) is invalid (0.0pt),
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 Package: nameref 2019/09/16 v2.46 Cross-referencing by name of section
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 )
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-LaTeX Info: Redefining \pageref on input line 61.
-LaTeX Info: Redefining \nameref on input line 61.
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 \@outlinefile=\write6
 \openout6 = `presentation.out'.
@@ -1266,14 +1258,14 @@ Package biblatex Info: Automatic encoding selection.
 Package biblatex Info: Trying to load bibliographic data...
 Package biblatex Info: ... file 'presentation.bbl' found.
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 (./presentation.nav)
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  []
 
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 File: Images/necker.pdf Graphic file (type pdf)
 <use Images/necker.pdf>
 
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 ]
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 File: umsa.fd 2013/01/14 v3.01 AMS symbols A
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 <use Images/translocation_10_14.pdf>
 
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+Overfull \vbox (26.27502pt too high) detected at line 113
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 File: Images/fragmentation.pdf Graphic file (type pdf)
 <use Images/fragmentation.pdf>
 
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+Overfull \vbox (17.1368pt too high) detected at line 128
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 [8
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 File: Images/assemblage.pdf Graphic file (type pdf)
 <use Images/assemblage.pdf>
 
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 File: Images/TRD_all.pdf Graphic file (type pdf)
 <use Images/TRD_all.pdf>
 
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 [14
@@ -1418,7 +1410,7 @@ Overfull \vbox (41.39737pt too high) detected at line 194
 File: Images/gviz_stacked/TRD-TLX1.pdf Graphic file (type pdf)
 <use Images/gviz_stacked/TRD-TLX1.pdf>
 
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+Overfull \vbox (36.39737pt too high) detected at line 206
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 [15
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 File: Images/gviz_stacked/TRD-LMO2.pdf Graphic file (type pdf)
 <use Images/gviz_stacked/TRD-LMO2.pdf>
 
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 [16
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 File: Images/gviz_stacked/TRD-TAL1.pdf Graphic file (type pdf)
 <use Images/gviz_stacked/TRD-TAL1.pdf>
 
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+Overfull \vbox (36.39737pt too high) detected at line 226
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 [17
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 File: Images/gviz_stacked/TRD-ZFP36L2.pdf Graphic file (type pdf)
 <use Images/gviz_stacked/TRD-ZFP36L2.pdf>
 
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 [18
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 File: Images/RAG_ZFP36L2.pdf Graphic file (type pdf)
 <use Images/RAG_ZFP36L2.pdf>
 
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+Overfull \vbox (36.39737pt too high) detected at line 247
  []
 
 [19
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 File: Images/RAG_ZFP36L2_mech.pdf Graphic file (type pdf)
 <use Images/RAG_ZFP36L2_mech.pdf>
 
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 [20
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 File: Images/RAG_ZFP_TREC_ZFP.pdf Graphic file (type pdf)
 <use Images/RAG_ZFP_TREC_ZFP.pdf>
 
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 [21
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  [22
 
 ]
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 [23
 
-] [24
+]
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+<Images/dko_zfp_surv.png>
+
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+[24
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+[25
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 ]
 \tf@nav=\write7
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  ) 
 Here is how much of TeX's memory you used:
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  71i,27n,121p,1904b,790s stack positions out of 5000i,500n,10000p,200000b,80000s
 
-Output written on presentation.xdv (24 pages, 100344 bytes).
+Output written on presentation.xdv (26 pages, 126604 bytes).

+ 13 - 5
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+ 50 - 19
presentation.tex

@@ -18,11 +18,10 @@
 %Graphics and Videos
 % https://tex.stackexchange.com/questions/89088/how-to-embed-video-and-animation-in-latex-and-latex-beamer-step-by-step
 \usepackage{graphicx} %The mode "LaTeX => PDF" allows the following formats: .jpg  .png  .pdf  .mps
-
 \usepackage{animate}
 
 
-\usepackage[utf8]{inputenc}
+%\usepackage[utf8]{inputenc}
 %\usetheme{Antibes}
 \usefonttheme{professionalfonts}
 
@@ -82,7 +81,9 @@
 \begin{frame}
 \frametitle{Introduction}
 \begin{itemize}
-  \item \textbf{Rationnel} : les fragments d'ADN capturés peuvent contenir des points de cassures si ils sont à une distance proche des sondes. La séquence de ces fragments étant chimérique, l'alignement sur le génome de référence ne permet pas d'observer les translocations.
+  \item \textbf{Rationnel} : les fragments d'ADN capturés peuvent contenir des points de cassures 
+  si ils sont à une distance proche des sondes. La séquence de ces fragments étant chimérique, 
+  l'alignement sur le génome de référence ne permet pas d'observer les translocations.
   \item \textbf{Objectifs} : détecter les reads chimériques et identifier les partenaires qui les composent.
   \item \textbf{Hypothèse} : il existe des translocations impliquant des partenaires inconnus.
   \item \textbf{Hypothèse alternative} : il existe des insertions de TREC.
@@ -274,7 +275,7 @@
       \includegraphics[width=\textwidth]{Images/RSS.pdf}
     \end{figure}
     \begin{itemize}
-      \item Très probable ré-insertion d'une boucle d'excision du \textit{TRD} (D2-D3).
+      \item Très probable \alert{ré-insertion d'une boucle d'excision} du \textit{TRD} (D2-D3).
       \item Cependant il semble que des translocations homozygotes doivent être éliminées (long-reads).
     \end{itemize}
   \end{center}
@@ -285,31 +286,61 @@
   \begin{table}
     \begin{tabular}[tl]{lccccr}
       \toprule
-      Nom & Clonotypage NGS & NOTCH1/FBXW7 & ETP & HOXA & Comm \\
+      \textbf{Nom} & \textbf{Clonotype} & \textbf{NOTCH1/FBXW7} & \textbf{ETP} & \textbf{HOXA} & \textbf{Comm} \\
       \midrule
-      RAGOT & 2 x $\delta$D2-$\delta$D3 & 1 & 0 & 1 & \\
-      TAHIR	& $\delta$D2-$\delta$D3 // GL	& 1	& 1	& 1 & \\
-      NEREE	& NA	& 1	& “immature”	& 0 & \\
-      2080178	& $\delta$D2-$\delta$J1 // $\delta$V1-$\delta$J2	& 0	& 0	& 0 & \\
-      HAOUZI	& $\delta$D2-$\delta$J1 // $\delta$V1-$\delta$J2	& 1	& 0	& 0	& e1a2 \\
-      JANOWSKA	& NA	& 1	& NA	& 0 & \\
+      RAGOT & 2 x $\delta$D2-$\delta$D3 & Q2398* & 0 & 1 & \\
+      TAHIR	& $\delta$D2-$\delta$D3 // GL	& P2514fs	& 1	& 1 & \\
+      NEREE	&	& FBXW7 R465H	& “immature”	& 0 & \\
+      2080178	& $\delta$D2-$\delta$J1 // $\delta$V1-$\delta$J2	& (NRAS)	& 0	& 0 & \\
+      HAOUZI	& $\delta$D2-$\delta$J1 // $\delta$V1-$\delta$J2	& P2443fs	& 0	& 0	& e1a2 \\
+      JANOWSKA	&	& T1602S	&	& 0 & \\
       \bottomrule
     \end{tabular}
   \end{table}
-  $\Rightarrow$ Clonotypes compatibles et prédominance de mutations sur la voie \textit{NOTCH1}
+  \begin{itemize}
+    \item Clonotypes compatibles
+    \item Prédominance de mutations \alert{PEST} sur la voie NOTCH1 ($\thickapprox 80\%$)
+  \end{itemize}
 \end{frame}
 
 
-\begin{frame}
-  \frametitle{ZFP36L2}
-  \begin{itemize}
-    \item Molécule se liant à certains ARNm (RBP) en 3' UTR.
-    \item Régule l'expression en favorisant la dégradation de certains ARNm.
-    \item dKO murin $\rightarrow$ LAL-T
-  \end{itemize}
+\begin{frame}[t]
+  \frametitle{ZFP36L2 --- Bibliographie }
+    \begin{columns}[onlytextwidth,T]
+      \column{\dimexpr\linewidth-30mm}
+      \begin{itemize}
+        \item Molécule de liaison aux ARNm (RBP) en 3' UTR.
+        \item Régule l'expression en favorisant la dégradation de certains ARNm (excision de la queue polyA).
+        \item \alert{dKO (L1$^{\textrm{fl}/\textrm{fl}}$L2$^{\textrm{fl}/\textrm{fl}}$ cd2-cre) murin $\rightarrow$ LAL-T}.
+        \item Réduction de l'expression d'icTCR-β dans la pop DN dKO.
+        \item Augmentation de l'expression de \alert{Notch1} relativement à thymus normal et notamment dans la pop DN.
+        \item L'expression de ZFP36L2 serait régulée positivement par Tcf-1.
+      \end{itemize}
+      \column{30mm}
+      \includegraphics[width=40mm]{Images/dko_zfp_surv.png}
+    \end{columns}
+  
   \blfootnote{Hodson DJ, Janas ML, Galloway A, et al. Deletion of the RNA-binding proteins ZFP36L1 and ZFP36L2 leads to perturbed thymic development and T lymphoblastic leukemia. Nat Immunol. 2010;11(8):717-724}
+  \blfootnote{Wang F, Qi Z, Yao Y, et al. Exploring the stage-specific roles of Tcf-1 in T cell development and malignancy at single-cell resolution. Cell Mol Immunol. 2021;18(3):644-659. doi:10.1038/s41423-020-00527-1}
 \end{frame}
 
+\begin{frame}[t]
+  \frametitle{ZFP36L2 --- Bibliographie}
+    \begin{itemize}
+      \item L'\alert{hyperméthylation} d'un super enhancer de ZFP36L2 est observée dans les cancers squameux de l'oesophage ($\thicksim$1 kb en aval du 3’UTR).
+      \item Des \alert{mutations récurrentes} de type fs ou ponctuelle ont été observées dans les LA. Jurkat = P190L, CEM et PEER = A329V. Ainsi que des délétions.
+      \item Dans LT$_{\textrm{M}}$ liaison aux pre-ARNm notamment de INFg, TNF, Pim1 puis \alert{bloque la formation du complexe ribosomal} et la traduction. Lors de l'activation des LT$_{\textrm{M}}$ cette \alert{inhibition est rapidement levée} et permet une traduction importante.
+    \end{itemize}
+  \blfootnote{Lin, De-Chen et al. “Identification of distinct mutational patterns and new driver genes in oesophageal squamous cell carcinomas and adenocarcinomas.” Gut vol. 67,10 (2018): 1769-1779. doi:10.1136/gutjnl-2017-314607}
+  \blfootnote{Iwanaga E, Nanri T, Mitsuya H, Asou N. Mutation in the RNA binding protein TIS11D/ZFP36L2 is associated with the pathogenesis of acute leukemia. Int J Oncol. 2011;38(1):25-31. }
+  \blfootnote{Salerno F, Engels S, van den Biggelaar M, et al. Translational repression of pre-formed cytokine-encoding mRNA prevents chronic activation of memory T cells. Nat Immunol. 2018;19(8):828-837. doi:10.1038/s41590-018-0155-6}
+\end{frame}
+
+\begin{frame}[standout]
+  Supp
+\end{frame}
+
+
 \end{document}
 
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