m

src/lib.rs

@@ -402,7 +402,7 @@ impl Sam {
         if is_reverse(flag) {
             let len = ref_range.len();
             ref_range.end = (ref_pos as usize) + 1;
-            ref_range.start = ref_pos as usize + len - 1; // wo -1 panic at 501 make_aln
+            ref_range.start = ref_pos as usize + len - 2; // wo -1 panic at 501 make_aln
             query_range.end = query_range.end - 1;
         } else { 
             let len = ref_range.len();